Polarization-resolved second-harmonic-generation optical coherence tomography in collagen
نویسندگان
چکیده
منابع مشابه
Polarization-resolved second-harmonic-generation optical coherence tomography in collagen.
We describe a novel imaging technique, second-harmonic-generation optical coherence tomography (SHOCT). This technique combines the spatial resolution and depth penetration of optical coherence tomography (OCT) with the molecular sensitivity of second-harmonic-generation spectroscopy. As a consequence of the coherent detection required for OCT, polarization-resolved images arise naturally. We d...
متن کاملPolarization-resolved second-harmonic generation in tendon upon mechanical stretching.
Collagen is a triple-helical protein that forms various macromolecular organizations in tissues and is responsible for the biomechanical and physical properties of most organs. Second-harmonic generation (SHG) microscopy is a valuable imaging technique to probe collagen fibrillar organization. In this article, we use a multiscale nonlinear optical formalism to bring theoretical evidence that an...
متن کاملHigh-resolution second-harmonic optical coherence tomography of collagen in rat-tail tendon
A high-resolution second-harmonic optical coherence tomography sSH-OCTd system is demonstrated using a spectrum broadened femtosecond Ti:sapphire laser. An axial resolution of 4.2 mm at the second-harmonic wave center wavelength of 400 nm has been achieved. Because the SH-OCT system uses the second-harmonic generation signals that strongly depend on the orientation, polarization, and local symm...
متن کاملAmplitude ambiguities in second-harmonic generation frequency-resolved optical gating.
We construct field shapes with distinct amplitude profiles that have nearly identical second-harmonic generation frequency-resolved optical gating (SHG FROG) traces. Although such fields are not true mathematical ambiguities, they result in experimentally indistinguishable FROG traces. These fields are neither time-reversed copies nor pulselets with a mere relative phase difference, which are w...
متن کاملHigh-resolution frequency-domain second-harmonic optical coherence tomography.
We used continuum generated in an 8.5 cm long fiber by a femtosecond Yb fiber laser to improve threefold the axial resolution of frequency domain second-harmonic optical coherence tomography (SH-OCT) to 12 microm. The acquisition time was shortened by more than 2 orders of magnitude compared to the time-domain SH-OCT. The system was applied to image biological tissue of fish scales, pig leg ten...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Optics Letters
سال: 2004
ISSN: 0146-9592,1539-4794
DOI: 10.1364/ol.29.002252